Next Generation Sequence Data Analysis Bootcamp

Curriculum Requirements Instructor Specifications

This course introduces protocols, necessary tools and programming techniques to handle — NGS data analysis. Theoretical background, underlying concepts behind every tool and methods will be explained along with procedural steps to get desired results from complex data types. Hands on with real word data facilitated by a team of Bioinformatics experts.

Curriculum

1.

Day 1: Linux workstation setup and basics. Most Bioinformatics tools and server environments are running in Linux. It will be extremely useful to get yourself familiar with Linux and command line tools.
2.

Day 1: Python basics. Due to it’s simple syntax and vast range of available modules, python is one of the most preferred languages in the Bioinformatics world.
3.

Day 1: R / Bioconductor basics. Most next generation sequencing platforms offer direct support via R modules. Data frames, annotations, package management.
4.

Day 2: RNA-seq expression analysis. Theoretical background, data preparations, statistical guidelines and visualization techniques using DESeq2.
5.

Day 3: WES Analysis. Theoretical background, reads preprocessing, alignment, post-processing, variant calling, SNV and Indel reporting.
6.

Day 4: WGS Analysis. Theoretical background, read quality assessment, alignment, variant calling, genome assembly, genome annotation, downstream analysis.
7.

Day 5: Visualization Techniques. Create complex heatmaps, integrative analysis reports, statistical visualizations, image readability and publication readiness.

Register

Please visit www.genix.ai/ngs-bootcamp and complete the registration.

Requirements

1.

Computer with Linux / Mac ( Virtual machine in case of windows )
It’s totally possible to use the Windows operating system, but using Unix will make it easier to adapt and communicate with the server. At least 4 GB of RAM and 10 GB Disk space is recommended.
2.

Good internet connection
We will not be downloading any large database into your system, but a good internet connection will help setup up programming modules easily and connection to server also depends on the internet connection.
3.

Molecular Biology basics
We expect some familiarity with the central dogma of molecular biology. We have limited time to cover the basics of what is DNA, RNA, transcription, translation and gene regulation.

Register

Please visit www.genix.ai/ngs-bootcamp and complete the registration.